pGLO Lab

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 400 yellow dull greenish yellow - pGLO LB/amp 0 no colonies no colonies + pGLO LB/amp 225 yellow dull greenish yellow + pGLO LB/amp/ara 200 yellow neon green

2.	What two new traits do your transformed bacteria have?
	The bacteria glows neon green and it has grown.
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic.
	On the LB/amp/ara, there was about 215, because there were about 200 colonies, and there are some hidden ones on the side. There was about 245 for LB/amp, and about 415 for LB because of the reasons above. There weren't any bacteria for LB/amp because there weren't any colonies.
4.	What is the role of arabinose in the plates?
	The sugar feeds the bacteria and helps it grow.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science.
	Cancer cells can be implemented with materials to make it glow. Then, these cells can be put in lab rats and be easily seen and easily tested on. GFP can also act as a biosensor or cell marker, or can be used for studying protein-protein interactions.
6.	Give an example of another application of genetic engineering. Genetic engineering can be used to mass produce human growth hormones and insulin.