Thinking Like a Biotechnician

1. I could use tetracycline to see if bacteria is taken in my plasmid. I could not use ampicillan antibiotics because that is what my plasmid is resistant to.
2. Restriction enzymes are enzymes that cut DNA whenever it reads a specific sequence. I used Eco RI because it fit once in my plasmid and twice in my cell DNA.
3. If I cut the plasmid twice, there would be two spaces for only one cell DNA section, and combined they would create a line instead of a circle.
4. In our everyday life, this process is important to help take out harmful genes and/or take out certain genes that are resistant to some antibiotics that are needed to cure or treat a disease.
5. Current technologies use recombinant DNA to clone animals (like "Dolly" the sheep) and to produce genetically modified foods.


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